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目的：探索视紫红质在鼠视网膜光化学损伤中的变化规律。方法：用微量可见紫外分光光度仪分别测定接受连续光照１ 、２ 、３ 、４ 天后以及光照１ 和３ 天，置暗环境恢复２ 、７ 、１４ 天后大鼠视网膜视紫红质含量的变化。结果：连续光照致视紫红质含量在光照开始的１ ～２ 天内急骤下降，以后趋于平缓。在一定范围内延长光照时间可使视紫红质含量水平更为低下。光照后暗环境中其含量的恢复并非呈直线上升，在１ 周左右表现为一个回落。结论：连续光照可使大鼠视网膜视紫红质含量显著减少；光照时间在一定范围内与含量水平密切相关。微量分光光度法为一种检测视网膜视紫红质含量的简便和快速的方法。
Objective: To explore the regularity of rhodopsin in photochemical damage of retina in rats. Methods: The contents of retinal rhodopsin in rat retinal detachment after 1, 2, 3 and 4 days of continuous light irradiation, 1 and 3 days of light exposure, and 2, 7 and 14 days of darkness recovery after exposure to continuous light were measured by using the micro-visible ultraviolet spectrophotometer. Results: The content of rhodopsin in continuous light rapidly decreased within 1 ~ 2 days after light began, and then became flat afterwards. Prolonging light time within a certain range can make rhodopsin levels even lower. The restoration of its content in the dark environment after light illumination did not show a straight rise, showing a drop in about 1 week. Conclusion: Continuous light can significantly reduce the content of retinal rhodopsin in rats; light exposure time is closely related to content level within a certain range. Trace spectrophotometry is a simple and rapid method for detecting retinal rhodopsin content.