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目的:ASICs通道及P/Q钙通道均参与偏头痛发生,分析ASICs通道及P/Q钙通道的电生理相互作用,评价二者的在偏头痛发生中的交互影响。方法:健康SPF级野生型C57BL/6鼠婴,分离培养双侧三叉神经节神经元,采用全细胞膜片钳技术记录三叉神经节神经元的钙电流变化及动作电位变化。结果:酸性外液及阿米洛利对钙通道无直接影响,酸性外液及P/Q通道阻断剂Aga-IVA均增加三叉神经元兴奋性(P<0.05),而阿米洛利可阻断这种增加效应(P<0.05)。结论:阿米洛利能够抑制Aga-IVA对三叉神经节神经元兴奋性的增加,可能与其阻断ASICs通道有关,提示ASICs通道可能为P/Q通道突变引发偏头痛的下游机制之一。
OBJECTIVE: ASICs channels and P / Q calcium channels are involved in the development of migraine. The electrophysiological interactions of ASICs channels and P / Q calcium channels were analyzed to evaluate the interaction between them. Methods: Bilateral trigeminal ganglion neurons were isolated and cultured in healthy SPF wild type C57BL / 6 mice. Whole cell patch clamp technique was used to record the change of calcium current and the change of action potential in trigeminal ganglion neurons. Results: Acidic extra-fluid and amiloride had no direct effect on calcium channel. Acidic external fluid and Aga-IVA, a P / Q channel blocker, increased the excitability of trigeminal neurons (P <0.05) Blocked this increase effect (P <0.05). CONCLUSION: Amiloride can inhibit the increase of excitability of trigeminal ganglion neurons by Aga-IVA, which may be related to its blockage of ASICs channels, suggesting that ASICs channels may be one of the downstream mechanisms of P / Q mutation triggering migraine.