目的:探讨慢病毒介导色素上皮衍生因子(n PEDF)基因修饰后人脐带间充质干细胞(hUCMSCs)的蛋白质组学变化。n 方法:将hUCMSCs分为基因修饰组和对照组，基因修饰组采用慢病毒介导n PEDF基因修饰hUCMSCs，对照组为正常hUCMSCs，提取各组细胞蛋白；采用FASP酶切法制备蛋白质样本，进行液相-质谱联用仪检测，采用SWATH模式采集数据。根据蛋白检测结果，进行差异蛋白分析及相应蛋白基因本体论(GO)分析和Reactome信号通路显著性富集分析。n 结果:共鉴定出5 361个可定量蛋白，与对照组相比，实验组慢病毒介导的n PEDF基因修饰后差异表达倍数>1.5且n P1.5,n P<0.05). There were 219 of the 432 proteins up-regulated, including serpin family F member 1 (SERPINF1) (PEDF), DEAD-box helicase 59 (DDX59) and thrombospondin 1 (THBS1), etc., whereas 213 proteins were down-regulated, including collagen type Ⅰ alpha 1 (COL1A1), COL18A1, etc.GO analysis indicated that the differential proteins were mainly involved in fibrinolysis, extracellular structure organization, regulation of transporter activity, biosynthetic process of secondary alcohol and cholesterol, coenzyme metabolic process and regulation of peptidase activity, etc.Reactome pathway analysis showed that the differential proteins were mostly involved in regulation of insulin like growth factor (IGF) transport and uptake by IGF binding protein, post-translational protein phosphorylation, extracellular matrix organization, metabolism of steroids.n Conclusions:After gene modification with n PEDF mediated by lentivirus, the expression of many proteins in hUCMSCs were changed.n PEDF gene modification can alter the structure of extracellular matrix and regulate the expression of proteins associated with cell proliferation, self-renewal and multipotency.n