【摘 要】
：
黄鳍棘鲷 (Acanthopagrus latus) 是中国具有重要商业价值的海水鱼类之一，对其精子开展冷冻保存可为黄鳍棘鲷育种提供一定的技术支持，可有效预防其种质资源衰退，保持其养殖业的可持续发展。该研究以黄鳍棘鲷精子为实验材料，对其精子冷冻保存的稀释液、葡萄糖浓度、抗冻剂种类及浓度、精子与保护液稀释比例和降温程序等进行了筛选优化。结果表明，以含10 g·L−1葡萄糖、5%乙二醇的MPRS溶液与精子按1∶2的比例稀释，4 ℃平衡30 min，液氮面上5 cm熏蒸5 min，最后投入液氮保存2 h，将黄鳍
【机 构】
：
河北农业大学海洋学院,河北,秦皇岛,066003;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东,广州,510300;广东省海洋生物种业工程技术研究中心,广东,广州,
论文部分内容阅读
黄鳍棘鲷 (Acanthopagrus latus) 是中国具有重要商业价值的海水鱼类之一，对其精子开展冷冻保存可为黄鳍棘鲷育种提供一定的技术支持，可有效预防其种质资源衰退，保持其养殖业的可持续发展。该研究以黄鳍棘鲷精子为实验材料，对其精子冷冻保存的稀释液、葡萄糖浓度、抗冻剂种类及浓度、精子与保护液稀释比例和降温程序等进行了筛选优化。结果表明，以含10 g·L−1葡萄糖、5%乙二醇的MPRS溶液与精子按1∶2的比例稀释，4 ℃平衡30 min，液氮面上5 cm熏蒸5 min，最后投入液氮保存2 h，将黄鳍棘鲷冷冻精子于37 ℃水浴解冻后活性最好，精子活力、运动时间和寿命可分别达到 (85.17±3.66)%、(9.16±7.70) s和 (94.29±9.55) s。“,”Acanthopagrus latus is one of the important breeding species in China, whose sperm cryopreservation can provide technical supports for its breeding, prevent the decline of its germplasm resources and maintain its sustainable development of the breeding industry effectively. In this experiment, we screened the diluent, glucose concentration, antifreeze type and concentration as well as dilution ratio and cooling procedure of the spermatozoa of A. latus. The results show that when the sperm were diluted with MPRS solution containing 10 g·L−1 glucose and 5% ethylene glycol at the ratio of 1∶2, balanced at 4 ℃ for 30 min, fumigated 5 cm on the liquid nitrogen surface for 5 min, and finally stored in liquid nitrogen for 2 h, the frozen sperm had the best motility after thawing in 37 ℃ water bath, and the sperm motility, motility time and life span could reach (85.170±3.66)%, (9.16±70) s and (94.297±9.55) s, respectively.
其他文献
Previous studies have shown that Lycium barbarum polysaccharide, the main active component of Lycium barbarum, exhibits anti-inflammatory and antioxidant effects in treating neurological diseases. However, the therapeutic action of Lycium barbarum polysac
Small Aδ and C nerve fibers of the sensory and autonomic nervous systems constitute 70–90％ of peripheral nerve fibers including corneal nerves (Muller et al., 2003).
Dexanabinol (HU-211) is an artificially synthesized cannabinoid derivative that exerts neuroprotective effects through anti-inflammatory and antioxidant effects. Curcumin exhibits antidepressant effects in the treatment of major depressive disorder. To in
Human neurological disorders and spinal cord injuries are caused by a loss of neurons and glial cells in the brain or spinal cord. The proof-of-principle of cell therapy is that replacing damaged cells with new healthy ones will restore the lost function.
Our previous studies showed that ferroptosis plays an important role in the acute and subacute stages of spinal cord injury. High intracellular iron levels and low glutathione levels make oligodendrocytes vulnerable to cell death after central nervous sys
Despite emerging contemporary biotechnological methods such as gene- and stemcell-based therapy, there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury. Our previous studies have demonstrated that trans
The axon initial segment (AIS) region is crucial for action potential initiation due to thepresence of high-density AIS protein voltage-gated sodium channels (Nav). Nav channels comprise several serine residues responsible for the recruitment of Nav chann
Human induced pluripotent stem cells (hiPSCs) are multipotent stem cells genetically reprogrammed using transcription factors, such as Sox2, c-Myc, Oct3/4 and Klf4 (Takahashi and Yamanaka, 2006) from fibroblasts, derived from either patient or control ind
Functional electrical stimulation is an effective way to rebuild hindlimb motor function after spinal cord injury. However, no site map exists to serve as a reference for implanting stimulator electrodes. In this study, rat models of thoracic spinal nerve