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1BL·1RS易位系在我国小麦育种和生产中占有重要地位,快速而准确地鉴定1BL·1RS易位系对小麦品质改良具有重要意义。本研究选用15个1BL·1RS特异性STS、SCAR和RAPD标记及22个1RS染色体上的SSR标记,检测不同来源的1BL·1RS易位系78份以及非1BL·1RS易位系品种10份和黑麦材料3份,其中1BL·1RS易位系包括周8425B及其衍生系36份、兰考906及其衍生系5份、矮孟牛及其衍生系8份和洛类1BL·1RS易位系品种29份。结果表明,7个STS标记、1个SCAR标记、3个RAPD标记和3个黑麦SSR标记可作为鉴别1BL·1RS易位系的可靠分子标记,其中ω-sec-p1/ω-sec-p2、ω-sec-p3/ω-sec-p4、H20和SECA2/SECA3标记最好,扩增效果稳定,重复性好,条带清晰,实验操作简单。周8425B及其衍生系、兰考906及其衍生系、矮孟牛及其衍生系与洛类1BL·1RS易位系的1RS染色体在分子标记检测中没有差异。
The 1BL · 1RS translocation system plays an important role in wheat breeding and production in China. The rapid and accurate identification of the 1BL · 1RS translocation line is of great significance to wheat quality improvement. In this study, 15 1BL · 1RS-specific STS, SCAR and RAPD markers and 22 SSR markers on 1RS chromosome were used to detect 78BLs derived from 1BL · 1RS translocation and 10BLFs from non-1BL · 1RS translocations 3 parts of rye material, among which 1BL · 1RS translocation line included 36 lines of Zhou 8425B and its derivative lines, 5 of Lankao 906 and its derivative lines, 8 of Ammunix and its derivatives and 1BL · 1RS 29 varieties. The results showed that seven STS markers, one SCAR marker, three RAPD markers and three rye SSR markers could be used as reliable molecular markers to identify the 1BL · 1RS translocation line, in which ω-sec-p1 / ω-sec-p2 The results showed that the amplification was stable, the repeatability was good, the bands were clear and the experimental operation was simple. Zhou 8425B and its derivatives, Lankao 906 and its derivatives, Ammonites and their derived lines and the 1RS · 1RS translocation line 1RS chromosome no difference in the detection of molecular markers.