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Bone marrow mesenchymal stem cells(MSCs) from rats were transfected with Rn-siRNA-caveolin-1 and differentiated into neuron-like cells using fasudil hydrochloride.Membrane ultrastructural changes in MSCs were observed under atomic force microscopy.Caveolin-1-transfected rat MSCs exhibited weak nuclear refraction,dense caveolae and long finger-like cellular processes prior to fasudil hydrochloride treatment.MSCs differentiating into neuron-like cells exhibited weak nuclear refraction and large cellular processes without caveolae.We hypothesize that caveolin-1 plays an important role in the regulation of bone marrow MSC differentiating into neuron-like cells.
Bone marrow mesenchymal stem cells (MSCs) from rats were transfected with Rn-siRNA-caveolin-1 and differentiated into neuron-like cells using fasudil hydrochloride. Membrane ultrastructural changes in MSCs were observed under atomic force microscopy. Caveolin-1-transfected rat MSCs express weak nuclear refraction, dense caveolae and long finger-like cellular processes prior to fasudil hydrochloride treatment. MSCs differentiating into neuron-like cells exhibiting weak nuclear refraction and large cellular processes without caveolae. We hypothesize that caveolin-1 plays an important role in the the regulation of bone marrow MSC differentiating into neuron-like cells.