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目的观察细胞角蛋白5/6(CK5/6)及14(CK14)在乳房外Paget病中的表达特点,并探讨其生物学意义。方法应用免疫组化二步法分别检测34例乳房外Paget病患者皮损及20例正常对照皮肤中CK5/6及CK14的表达情况。结果 CK5/6和CK14在乳房外Paget病中阳性表达,两者主要在表皮全层弥漫性表达,表达率分别为91.17%和88.24%。结论 CK5/6和CK14在乳房外Paget病的组织来源方面起到一定的启示作用,推测乳房外Paget病的肿瘤细胞可能来源于异常分化的基底细胞层表皮干细胞。
Objective To observe the expression of cytokeratin 5/6 (CK5 / 6) and 14 (CK14) in extramammary Paget’s disease and to explore its biological significance. Methods The expression of CK5 / 6 and CK14 in 34 cases of extramammary Paget disease and 20 cases of normal control skin were detected by immunohistochemical two-step method. Results CK5 / 6 and CK14 were positive in extramammary Paget’s disease. The two were diffusely expressed in the whole epidermis, with the expression rates of 91.17% and 88.24% respectively. Conclusions CK5 / 6 and CK14 play an important role in revealing the origin of extramammary Paget’s disease. It is speculated that extramammary Paget’s tumor cells may originate from abnormally differentiated basal cell layer epidermal stem cells.