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目的:观察血液灌流(HP)对灭多威中毒兔损伤心肌的保护作用及其机制。方法:24只兔随机分为3组:正常对照组(N组)、中毒组(P组)、血液灌流组(HP组),每组8只,P组灭多威中毒模型,假血液灌流2h,HP组兔灭多威中毒模型,行血液灌流2 h,观察光镜下心肌组织的病理学改变特点,采用比色法检测各组兔心肌组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,应用SPSS16.0软件对实验所得数据进行统计。结果:光学显微镜观察下,P组心肌组织可见充血、水肿等病理学改变,HP组与N组心肌组织充血水肿等病理改变不明显;与N组,HP组比较,P组实验兔心肌组织SOD活性明显下降,差异有统计学意义(P<0.05),P组MDA含量有明显升高差异具有统计学意义(P<0.05)。结论:早期血液灌流可减轻灭多威中毒所致心肌组织损伤,其机制与血液灌流的抗氧化损伤有关。
Objective: To observe the protective effect and mechanism of hemoperfusion (HP) on myocardial injury induced by methomyl in rabbits. Methods: Twenty-four rabbits were randomly divided into three groups: normal control group (N group), poisoning group (P group) and hemoperfusion group (HP group), with 8 mice in each group. The rabbits were sacrificed at 2 hours, and then treated with hemoperfusion for 2 hours. The pathological changes of myocardium under light microscope were observed. The activity of superoxide dismutase (SOD) in myocardium was detected by colorimetry. Dialdehyde (MDA) content, the application of SPSS16.0 software on the experimental data statistics. Results: Pathological changes such as hyperemia and edema were observed in the myocardial tissue of P group under optical microscope. The pathological changes of hyperemia and edema in myocardial tissue of HP group and N group were not obvious. Compared with N group and HP group, (P <0.05). The content of MDA in group P was significantly increased (P <0.05). Conclusion: Early hemoperfusion can reduce the myocardial tissue damage caused by methomyl poisoning, and its mechanism is related to the anti-oxidative damage of hemoperfusion.