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目的观察鱼藤酮对帕金森模型大鼠各脏器的损伤,探讨牛蒡苷元对其缓解与治疗作用。方法采用颈背部皮下注射鱼藤酮葵花油乳化液4周的方法建立大鼠帕金森模型,并灌胃给予不同剂量的牛蒡苷元进行干预治疗,观察各组大鼠脏器指数。采用HE染色法和电镜法观察大鼠脏器损伤程度,检测血清中丙氨酸转移酶(alanine aminotransferase,ALT)、肌酐(creatinine,CRE)与尿素氮(urea nitrogen,BUN)的含量。结果模型组大鼠的肝指数和肾指数与正常组相比显著升高,血清中ALT与BUN的含量显著升高(P≤0.01),肝脏切片显示肝脏内存在着中央静脉扩张、慢性炎症细胞浸润等病理改变,肾皮质和髓质内均有慢性出血和炎性浸润,并可见肾小球固缩。牛蒡苷元治疗后肝肾指数显著降低,血清中ALT与BUN明显降低(P≤0.01),肝肾相应损伤有所缓解。结论牛蒡苷元能有效抑制鱼藤酮所致的大鼠肝脏和肾脏肿大,可缓解和治疗鱼藤酮所引起肝和肾的损伤。
Objective To observe the damage of rotenone to various organs of Parkinson’s model rats and to discuss the effect of arctigenin on its alleviation and treatment. Methods Parkinson ’s disease model was established by subcutaneous injection of rotenone sunflower oil emulsion on the back of the neck for 4 weeks. The rats were given intragastric administration of different doses of arctigenin for intervention. The indexes of the organs in each group were observed. The degree of organ damage was observed by HE staining and electron microscopy. The levels of serum alanine aminotransferase (ALT), creatinine (CRE) and urea nitrogen (BUN) were measured. Results The liver index and renal index in model group were significantly higher than those in normal group, the levels of ALT and BUN in serum were significantly increased (P≤0.01), and the liver slices showed the presence of central venous dilatation in liver. Chronic inflammatory cells Invasion and other pathological changes, renal cortex and medulla have chronic hemorrhage and inflammatory infiltration, and visible glomerular condensation. Arctigenin treatment significantly reduced liver and kidney index, serum ALT and BUN was significantly lower (P ≤ 0.01), liver and kidney damage was eased. Conclusion Arctigenin can effectively inhibit the enlargement of rat liver and kidney caused by rotenone and can alleviate and treat the damage of liver and kidney caused by rotenone.