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目的:建立同时测定加味逍遥提取物中6个指标性成分(栀子苷、芍药内酯苷、芍药苷、甘草苷、苯甲酰芍药苷、甘草酸)含量方法。方法:采用超高效液相色谱法,以UPLC~(TM)HSS T_3色谱柱(2.1 mm×100 mm,1.8μm),乙腈-0.05%磷酸水溶液为流动相梯度洗脱,柱温30℃,体积流量0.4 m L·min~(-1),检测波长230 nm。结果:6个指标性成分分离度良好,均在各自线性范围内线性关系良好,R2≥0.999 4;平均加样回收率为98.29%~101.67%,RSD均<3.8%。结论:该法准确、简便、分离度良好,可作为加味逍遥提取物的质量评价方法。
Objective: To establish a method for the simultaneous determination of 6 index components (Geniposide, Paeoniflorin, Paeoniflorin, Glycyrrhizin, Benzoyl paeoniflorin and Glycyrrhizin) in Jiawei Xiaoyao extract. METHODS: The mobile phase was eluted with UPLC-HSS T_3 column (2.1 mm × 100 mm, 1.8 μm) and acetonitrile-0.05% phosphoric acid aqueous solution using ultra performance liquid chromatography with a column temperature of 30 ℃ and volume The flow rate was 0.4 m L · min ~ (-1) and the detection wavelength was 230 nm. Results: The six index components were well separated and had good linearity in their respective linear ranges with R2≥0.9994. The average recovery rates were 98.29% ~ 101.67% with RSDs less than 3.8%. Conclusion: The method is accurate, simple and good separation, which can be used as a quality evaluation method of Jiawei Xiaoyao extract.