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目的建立高效液相色谱法(HPLC法)测定连钱草中熊果酸和齐墩果酸含量的方法。方法依利特C18柱(4.6 mm×250 mm,5μm)为色谱柱,甲醇-0.05 M磷酸二氢钠溶液(87:13)为流动相,流速1.0 ml/min,检测波长210 nm。结果熊果酸在0.55~5.45μg范围内线性关系良好(r=0.9993),平均回收率98.32%(RSD=1.74%);齐墩果酸在0.22~2.19μg范围内线性关系良好(r=0.9996),平均回收率99.86%(RSD=1.84%)。结论 HPLC法简便准确,可为连钱草的质量控制提供实验依据。
OBJECTIVE To establish a method for the determination of ursolic acid and oleanolic acid in giancao by high performance liquid chromatography (HPLC). Methods Elite C18 column (4.6 mm × 250 mm, 5 μm) was used as the column. Methanol-0.05 M sodium dihydrogen phosphate (87:13) was used as the mobile phase at a flow rate of 1.0 ml / min and the detection wavelength was 210 nm. Results The ursolic acid showed a good linearity (r = 0.9993) in the range of 0.55-5.45μg with an average recovery of 98.32% (RSD = 1.74%). Oleanolic acid had a good linear relationship in the range of 0.22-2.19μg (r = 0.9996 ), The average recovery was 99.86% (RSD = 1.84%). Conclusion HPLC method is simple and accurate, which can provide experimental evidence for quality control of.