论文部分内容阅读
目的评价市售肠道病毒71型、柯萨奇A16-IgM胶体金试剂在临床手足口病诊断方面的应用。方法收集临床诊断为手足病发病7 d内的患儿血清标本658份,对患儿进行流行病学调查,明确感染日期和病程,利用市售肠道病毒71型、柯萨奇A16-IgM胶体金试剂检测IgM。结果共采集标本658份,肠道病毒阳性78份,阳性率为11.85%,其中男性患儿41例,女性患儿37例,男、女之比为1.11∶1。56份柯萨奇A16型、肠道病毒71型均出现阳性反应,交叉阳性结果达到71.79%(56/78)。4月、6月发病率较高,分别为16.35%、15.85%。各年龄段中,1岁~组患儿发病率最高,为37.18%。发病3 d后IgM抗体检出率最高,为28.21%。结论本试剂敏感性偏低,特异性较差,不适合临床早期诊断手足口病病原。
Objective To evaluate the clinical application of enterovirus 71 and Coxsackie A16-IgM colloidal gold in the diagnosis of clinical HFMD. Methods A total of 658 serum samples from children diagnosed as foot and / or shoulders disease were collected. The epidemiological investigation was performed on children and the date and duration of infection were determined. Commercial enterovirus 71, Coxsackie A16-IgM colloid Gold reagent detection of IgM. Results A total of 658 specimens were collected and 78 were positive for enterovirus. The positive rate was 11.85%. Among them, 41 cases were male and 37 cases were female. The ratio of male to female was 1.11:1.56 Coxsackie A16 , 71 were enterovirus positive reaction, cross-positive results reached 71.79% (56/78). In April and June, the incidence rates were higher, accounting for 16.35% and 15.85% respectively. In all age groups, 1-year-old group had the highest incidence rate of 37.18%. The incidence of IgM antibody was the highest at 3 d after onset, which was 28.21%. Conclusion This reagent has low sensitivity and poor specificity and is not suitable for early diagnosis of HFMD in clinic.