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为明确杀菌剂对玉米螟赤眼蜂Trichogramma ostriniae Pang et Chen毒性大小及其成蜂体内酚氧化酶(phenoloxidase,PO)活性影响,本实验通过测定三唑酮(triadimefon)、肟菌脂(trifloxystrobin)、咪鲜胺(prochloraz)、申嗪霉素(phenazino-1-carboxylic acid)4种杀菌剂对玉米螟赤眼蜂的急性毒性,并分别测定活体和离体条件下各杀菌剂处理后酚氧化酶的比活性。结果表明,各药剂对赤眼蜂的急性毒性大小为:三唑酮>咪鲜胺>肟菌酯>申嗪霉素,其LC50值分别为3.27,6.46,4.06和9.72mg/L,其安全系数依次为0.07,0.10,0.20和1.47,其中申嗪霉素为中等风险性,其余3种药剂为高风险性,且杀菌剂明显激活了PO的活性。活体条件下亚致死剂量处理得到的PO比活力高于离体处理,且三唑酮处理组具有明显的剂量效应。本实验为研究杀菌剂对赤眼蜂的毒性及对PO的影响作了初步的探索,为进一步研究赤眼蜂对杀菌剂的抗性及免疫能力奠定基础。
In order to clarify the effect of bactericide on Trichogramma ostriniae Pang et Chen toxicity and the activity of phenoloxidase (PO) in Trichogramma ostriniae, the effect of triadimefon, trifloxystrobin, Prochloraz and phenazino-1-carboxylic acid against Trichogramma ostriniae were measured. The effects of four bactericides on phenoloxidation Specific activity of the enzyme. The results showed that the acute toxicities of each agent to Trichogramma were: triadimefon> prochloraz> trifloxystrobin> sodiunmycin with LC50 values of 3.27, 6.46, 4.06 and 9.72 mg / L, respectively, The coefficients were 0.07, 0.10, 0.20 and 1.47, respectively. Among them, the nematocin was moderate risk and the other three agents were high risk, and the fungicide obviously activated the activity of PO. Under living conditions, the specific activity of PO induced by sublethal treatment was higher than that of ex vivo treatment, and the triadimefon treatment group had obvious dose effect. This experiment for the study of fungicides on Trichogramma toxicity and the impact of PO made a preliminary exploration for the further study Trichogramma biocide resistance and immunocompetence lay the foundation.