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【目的】建立高血压 -血管性痴呆 (VD)的动物模型 ,为研究其机理及中医证型提供形态学和行为学依据。【方法】在 1 0 0只 SD大鼠中随机取 88只制成肾性高血压 (RHR) ,1 2只作假术组 ;于第 36～42天进行水迷宫测试。在存活的 74只肾性高血压大鼠 (RHR)中 ,随机取 5 0只于第 43天阻断双侧颈总脉造成脑反复缺血再灌注损害。于第 5 6天进行水迷宫测试 ,取假术组游到平台平均时间的 95 %上限值为标准 ,将超出上限值的缺血 RHR确定为复合痴呆大鼠。【结果】存活的 39只缺血 RHR大鼠中 ,有 34只为痴呆大鼠 ,痴呆率达 87.1 8% (34 / 39)。至第 83天 ,模型组仍处于痴呆的水平。痴呆大鼠先后表现为肝阳上亢、肝肾不足、气血两虚、肾髓不足等证候。【结论】所建立的 VD复合模型 ,可用于对 VD进行较长时期的慢性研究 ;临床上治疗 VD患者时必须动态辨证。
【Objective】 To establish an animal model of hypertension-vascular dementia (VD) and provide morphological and behavioral evidence for studying its mechanism and TCM syndromes. 【Methods】 One hundred and eighty SD rats were randomized to receive renovascular hypertension (RHR). One hundred and two rats were randomly divided into sham operation group. Water maze test was performed on day 36-42. In 74 surviving renal hypertensive rats (RHR), 50 randomly selected rabbits were sacrificed on day 43 and blocked bilateral common carotid artery to cause cerebral ischemia-reperfusion injury. The water maze test was performed on the 56th day, and the upper limit of 95% of the average time for swimming to the platform in the sham group was taken as the standard. The ischemic RHR exceeding the upper limit was determined as a compound dementia rat. 【Results】 Out of 39 surviving RHR rats, 34 were dementia rats with dementia rate of 87.1% (34/39). By day 83, the model group was still at dementia level. Dementia rats have manifested as liver yang, liver and kidney deficiency, qi and blood deficiency, kidney deficiency and other syndromes. 【Conclusion】 The established VD composite model can be used for a long period of chronic VD study; clinical treatment of VD patients must be dynamic syndrome differentiation.