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[Objective] To study the culture conditions for callus and tube seedlings of Leibnitzia anandria L. [Method] With the flower stalks of L. anandria as explants, the method of tissue culture was adopted to investigate callus induction and differentiation, differentiation and multiplication of adventitious buds, rooting and transplanting of tube seedlings. [Result] MS+0.5 mg/L 6-BA +2.5 mg/L 2,4-D was the best medium for induction of callus. The optimum medium for the differentiation of callus as well as the differentiation and multiplication of adventitious buds was MS+0.8 mg/L AgNO3+0.1 mg/L NAA+1.2 mg/L 6-BA; the best rooting culture method of tube seedlings was inoculating the adventitious buds into 1/2MS+NAA 0.1 mg/L after being treated with 1.2 mg/L IAA for 24 h; the average survival rate of transplanting was 92.8% and survival rate of field planting was 97.7%. [Conclusion] The tube seedlings survived retained all botanical characteristics of the wild L. anandria with exuberant growth, and could be used for proliferation.
[Objective] To study the culture conditions for callus and tube seedlings of Leibnitzia anandria L. [Method] With the flower stalks of L. anandria as explants, the method of tissue culture was adopted to investigate callus induction and differentiation, differentiation and multiplication of adventitious buds, rooting and transplanting of tube seedlings. [Result] MS + 0.5 mg / L 6-BA + 2.5 mg / L 2,4-D was the best medium for induction of callus. The optimum medium for the differentiation of callus as well as the differentiation and multiplication of adventitious buds was MS + 0.8 mg / L AgNO3 + 0.1 mg / L NAA + 1.2 mg / L 6-BA; the best rooting culture method of tube seedlings was inoculating the adventitious buds into 1 / 2MS + NAA 0.1 mg / L after being treated with 1.2 mg / L IAA for 24 h; the average survival rate of transplanting was 92.8% and survival rate of field planting was 97.7%. [Conclusion] The tube seedlings survived retained all botanical characteristics of the wild L. anandria with exuberant grow th, and could be used for proliferation.