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目的:观察蝎毒多肽B5(SVPB5)对正常及辐射后的大鼠骨髓间充质干细胞(r BMSCs)生长的影响。方法:利用全骨髓细胞贴壁法分离纯化r BMSCs,体外扩增传代,取第3~5代细胞为实验对象。实验分为正常对照组(IR-control)、正常加药组(IR-SVPB5)、辐射对照组(IR+control)以及辐射加药组(IR+SVPB5)。分别进行细胞计数、衰老相关β半乳糖苷酶染色及集落形成单位计数。结果:SVPB5对正常及辐射r BMSCs均有促进增殖的作用,其中SVPB5处理3 d后,对正常r BMSCs开始便表现为明显的促增殖作用(P<0.05),而对辐射r BMSCs则于第10、11天表现出明显的促增殖作用(P<0.05)。SVPB5作用于正常r BMSCs后,其衰老染色阳性细胞率明显低于正常对照组(P<0.05),其集落数明显高于正常对照组(P<0.05)。结论:SVPB5能促进正常及辐射后r BMSCs增殖,增强r BMSCs的增殖潜能并缓解其衰老。
Objective: To observe the effects of scorpion venom peptide B5 (SVPB5) on the growth of rat bone marrow mesenchymal stem cells (rBMSCs) under normal and radiation conditions. Methods: BMSCs were isolated and purified by whole bone marrow adherent method and passaged in vitro. The 3rd to 5th passage cells were used as experimental subjects. The experiment was divided into three groups: IR-control group, IR-SVPB5 group, IR + control group and IR + SVPB5 group. Cell counts, senescence-related beta-galactosidase staining and colony forming units were counted. Results: SVPB5 could promote the proliferation of both normal and irradiated r BMSCs. SVPB5 treatment for 3 days showed a significant effect on proliferation of normal r BMSCs (P <0.05) 10 and 11 days showed a significant role in promoting proliferation (P <0.05). After treated with SVPB5, the percentage of senescent cells was significantly lower than that of normal control group (P <0.05), and the number of colony was significantly higher than that of normal control group (P <0.05). Conclusion: SVPB5 can promote the proliferation of normal and irradiated r BMSCs, enhance the proliferation potential of r BMSCs and relieve the aging of r BMSCs.