论文部分内容阅读
目的探讨细胞因子IL-22对小鼠输卵管上皮细胞趋化因子CXCL9、CXCL10表达的影响。方法流式细胞术检测体外培养的小鼠输卵管上皮细胞膜表面IL-22R表达水平。重组鼠IL-22(100 ng/ml)刺激小鼠输卵管上皮细胞,ELISA检测不同时间(0、12、24、48、72 h)CXCL9、CXCL10表达情况,Trans AM ELISA检测转录因子STAT3 DNA结合活性。不同质量浓度的重组鼠IL-22(0、25、50、100、200 ng/ml)刺激小鼠输卵管上皮细胞,ELISA检测CXCL9、CXCL10表达情况。结果小鼠输卵管上皮细胞膜表面表达IL-22R。比较6个时间点CXCL9、CXCL10表达差异,发现CXCL9、CXCL10表达具有时间依赖性。比较不同质量浓度IL-22处理下CXCL9、CXCL10表达差异,IL-22 100 ng/ml为最佳干预浓度,且CXCL9、CXCL10表达与IL-22质量浓度具有依赖关系。IL-22刺激组STAT3 DNA结合活性显著升高,与阴性对照组比较有显著差异(P<0.05)。结论 IL-22能显著诱导小鼠输卵管上皮细胞生成并释放CXCL9、CXCL10,且具有时间和剂量依赖性。
Objective To investigate the effect of cytokine IL-22 on the expression of chemotactic factors CXCL9 and CXCL10 in mouse oviduct epithelial cells. Methods Flow cytometry was used to detect the expression of IL-22R on the surface of mouse oviduct epithelial cells cultured in vitro. The mouse oviduct epithelial cells were stimulated with recombinant murine IL-22 (100 ng / ml). The expression of CXCL9 and CXCL10 at different time points (0, 12, 24, 48 and 72 h) was detected by ELISA. The transcript STAT3 DNA binding activity . The mouse oviduct epithelial cells were stimulated with different concentrations of recombinant murine IL-22 (0, 25, 50, 100, 200 ng / ml). The expression of CXCL9 and CXCL10 was detected by ELISA. Results The surface of mouse oviduct epithelial cells expressed IL-22R. Comparing the expression of CXCL9 and CXCL10 at 6 time points, it was found that the expression of CXCL9 and CXCL10 was time-dependent. The difference of expression of CXCL9 and CXCL10 between different concentrations of IL-22 was compared. IL-22 100 ng / ml was the best intervention concentration, and the expression of CXCL9 and CXCL10 was dependent on the concentration of IL-22. The STAT3 DNA binding activity of IL-22 stimulation group was significantly increased compared with the negative control group (P <0.05). Conclusion IL-22 can significantly induce the generation of oviduct epithelial cells and release CXCL9 and CXCL10 in a time-and dose-dependent manner.