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目的:验证Vere Flu芯片检测H7N9亚型流感病毒的特异性和准确性,为应对流感疫情提供新的实验室检测方法。方法:对流感病毒H7N9亚型、H5N1亚型、H1N1亚型的病毒培养物及10份H7N9感染疫区的鸡肛拭子标本,提取样本的RNA,采用Vere Flu芯片法和Realtime RT-PCR两种检测方法。评估Vere Flu芯片的检测效果。结果:对于3种病毒培养物的检测,Vere Flu芯片均检测到不同亚型的特定阳性信号,每种亚型病毒均未出现交叉反应信号,表明Vere Flu芯片具有较好的检测特异性;10份未知标本中有8份检测流感病毒阴性,2份检测流感病毒阳性,编号为106号及503号,其中106号检测到H7及H9亚型的阳性信号,503号仅检测到H9亚型的阳性信号。Realtime RT-PCR对10份未知标本进行H7亚型核酸检测,从两份流感阳性标本中均检测到H7亚型核酸,高通量测序证实106号和503号标本中均存在H7及H9亚型核酸。结论:Vere Flu芯片可用于不同型别流感病毒的检测和分型,包括新爆发的H7N9亚型病毒同样适用,但检测H7N9亚型的灵敏度低于Realtime RT-PCR法。
Objective: To verify the specificity and accuracy of Vere Flu chip in detecting H7N9 subtype influenza virus and to provide a new laboratory test method to deal with the flu epidemic. Methods: The virus samples of H7N9 subtype, H5N1 subtype, H1N1 subtype of influenza virus and 10 samples of chicken rectal swab infected with H7N9 were extracted and the RNA was extracted. The Vere Flu-chip and Realtime RT-PCR Species detection method. Evaluate the detection of Vere Flu chips. Results: For the detection of three virus cultures, Vere Flu chips detected specific positive signals of different subtypes, and no cross-reaction signal was observed in each subtype of virus, indicating that Vere Flu chip has good detection specificity. 10 Of the unknown samples, 8 were negative for influenza virus detection and 2 were positive for influenza virus detection, numbered 106 and 503, of which 106 were positive for H7 and H9 subtypes and 503 were only for H9 subtype Positive signal. H7 subtype nucleic acid was detected by Realtime RT-PCR in 10 unknown samples. H7 subtype nucleic acids were detected in two influenza positive samples. H7 and H9 subtypes were confirmed by high-throughput sequencing in both samples 106 and 503 Nucleic acid. Conclusion: The Vere Flu chip can be used for the detection and typing of different types of influenza viruses, including the newly discovered H7N9 subtype virus. However, the sensitivity of detecting H7N9 subtype is lower than that of Realtime RT-PCR.