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用肺泡灌洗法获取兔肺泡巨噬细胞,进行体外培养,取其上清液分别用同位素掺入法和MTT比色法测定上清液中炎性细胞因子TNF和IL-6的活性。并将上清液与人胚肺成纤维细胞(WI-38)培养,用同位素掺入法测定成纤维细胞生长增殖和胶原合成。用氯氨T法分析肺成纤维细胞总羟脯氨酸含量,并用抗TNF抗体和IFNγ进行成纤维细胞增殖和胶
Rabbit alveolar macrophages were obtained by alveolar lavage and cultured in vitro. The supernatants were harvested for the determination of the activity of inflammatory cytokines TNF and IL-6 by isotope incorporation method and MTT colorimetry, respectively. Supernatants were cultured with human embryonic lung fibroblasts (WI-38), and fibroblast growth and proliferation and collagen synthesis were measured by isotope incorporation. The total hydroxyproline content of lung fibroblasts was analyzed by the method of chloramine T, and fibroblast proliferation and adhesive were detected by anti-TNF antibody and IFNγ