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目的 探讨癌转移抑制基因nm2 3 H1 在人肺癌中的突变情况 ,及其与肺癌发生、发展及转移的关系。方法 采用聚合酶链反应—单链构象多态性技术 (PCR SSCP) ,对手术切除的 45例肺癌组织和 7例正常肺组织的nm2 3 H1 基因的 5个外显子进行突变分析。结果 在所检测的肺组织中 ,未发现nm2 3 H1 基因纯合缺失。SSCP分析发现一例肺癌组织nm2 3 H1 基因外显子 1单链DNA迁移率发生改变。此例患者为晚期肺鳞癌 ,伴有纵隔淋巴结转移和恶性胸水。结论 本实验所检测的nm2 3 H1 基因外显子部分的基因突变在肺癌中发生率低 ,nm2 3 H1 基因突变可能与肺癌进展及转移有关
Objective To investigate the mutation of the metastasis suppressor gene nm23H1 in human lung cancer and its relationship with the occurrence, development and metastasis of lung cancer. METHODS: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze mutations in the exon 5 of the nm23H1 gene in 45 lung cancer tissues and 7 normal lung tissues. Results No homozygous deletion of the nm23H1 gene was found in the examined lung tissues. SSCP analysis revealed that the migration rate of exon 1 single-stranded DNA of the nm23H1 gene in a lung cancer tissue was changed. This patient had advanced lung squamous cell carcinoma with mediastinal lymph node metastasis and malignant pleural effusion. Conclusion The gene mutations in the exon part of the nm23H1 gene detected in this experiment have a low incidence in lung cancer. The nm23H1 gene mutation may be associated with lung cancer progression and metastasis.