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钾是柑橘生长发育过程中必需的元素。At CIPK23在拟南芥的钾吸收利用过程中发挥了重要作用,在水稻、杨树和葡萄等多种植物中也发现了与At CIPK23功能相似的CIPK基因,说明类At CIPK23基因在植物进化过程中具有保守性。本研究从甜橙基因组序列信息中分析得到17个柑橘CIPK基因,将其中与At CIPK23基因进化关系较近的Cs2g08190、Cs2g28990和Orange1.1t00555作为柑橘类At CIPK23候选基因;候选基因编码产物与拟南芥CIPK家族成员的氨基酸序列相似性分析结果显示,Cs2g08190与At CIPK23同源性最高(76.8%);三个候选基因均能在细胞质定位,但仅发现Cs2g08190的表达受低钾胁迫诱导表达,推测Cs2g08190是柑橘类At CIPK23基因。
Potassium is an essential element in citrus growth and development. At CIPK23 played an important role in the potassium absorption and utilization of Arabidopsis. CIPK gene similar to At CIPK23 was also found in many plants such as rice, poplar and grape, indicating that At CIPK23 gene has the function of Conservative. In this study, 17 citrus CIPK genes were analyzed from sweet orange genome sequence information, and Cs2g08190, Cs2g28990 and Orange1.1t00555, which are closely related to the evolution of At CIPK23, were used as candidate citrus At CIPK23 candidate genes. The amino acid sequence similarity analysis of CIPK family members showed that Cs2g08190 had the highest homology (76.8%) with At CIPK23. All three candidate genes could be located in the cytoplasm. However, Cs2g08190 expression was induced by low potassium stress only Cs2g08190 is a citrus At CIPK23 gene.