通过透射电镜观察体外培养细胞 ,最早的方法是将细胞培养在载玻片上 ,按照顶扣法制备样品即可得到满意的结果[1] 。现在大部分体外培养的细胞生长在玻璃培养瓶中 ,需要用硅胶刮子将细胞刮下来然后离心 ,再按常规方法制作超薄切片才能在电镜下观察[2 ] 。有些细胞 ,如在塑料培养 The first method of culturing cells in vitro by transmission electron microscopy was to culture the cells on a glass slide and obtain satisfactory results by top-draw method [1]. Now most of the cells cultured in vitro are grown in glass flasks. They need to be scraped off with silica gel scraper and centrifuged. Ultrathin sections can be made by conventional methods to observe under electron microscopy [2]. Some cells, such as in plastic culture