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本文报道对64只豚鼠的血淋巴细胞,分别在电针、给予纳洛酮或外源性阿片样肽(OLP)等不同条件下,以PAP免疫组织化学法显示甲脑啡肽(MEK)、亮脑啡肽(LEK)及β-内啡肽(β-EP)的免疫反应(IR)。另对部分免疫组织化学标本,随后显示酸性非特异性酯酶(ANAE)。约96％的血淋巴细胞呈强弱不等的OLP阳性反应。甲脑啡肽强阳性反应淋巴细胞(MEK-IIRL)和β-EP强阳性反应淋巴细胞,与ANAE的点型淋巴细胞,均呈显著正相关;但LEK强阳性反应淋巴细胞与ANAE的点型淋巴细胞的相关不显著。电针豚鼠“足三里”穴后,MEK、LEK及β-EP的强阳性反应淋巴细胞计数均显著提高。将电针组、纳洛酮组和对照组的结果做比较,见3组间的OLP(包括脑啡肽和内啡肽)的强阳性反应淋巴细胞计数差别皆不显著。但当分别加10~(-7)mol/L MEK、LEK和β-EP体外孵育淋巴细胞后,纳洛酮对OLP强阳性反应淋巴细胞有抑制效应,纳洛酮组和电针组的OLP强阳性反应淋巴细胞计数均呈显著差异。在外加10~(-12)～10~(-3)mol/L MEK体外孵育淋巴细胞后,可见电针组比对照组在10~(-10)～10~(-3)mol/L浓度间MEK免疫反应呈现一个明显高峰,提示电针可能提高潜在未结合的MEK受体的活性。
In this paper, it is reported that the blood lymphocytes of 64 guinea pigs were treated with electroacupuncture, naloxone, or exogenous opioid peptide (OLP), and anesthesia (MEK) was shown by PAP immunohistochemistry. Enkephalin (LEK) and β-endorphin (β-EP) immunoreactivity (IR). In addition, a partial immunohistochemical specimen was followed by an acidic non-specific esterase (ANAE). About 96% of the blood lymphocytes showed positive and negative OLP positive reactions. Strong positive reaction lymphocytes (MEK-IIRL) and β-EP positive reaction lymphocytes of leukoenkephalin were positively correlated with the dot type lymphocytes of ANAE; however, the strong positive reaction lymphocytes with LEK and ANAE were of point type. The correlation of lymphocytes is not significant. Electroacupuncture at the “Zusanli” point in guinea pigs significantly increased the lymphocyte counts of MEK, LEK and β-EP. Comparing the results of the EA group, the naloxone group, and the control group, the difference in lymphocyte counts between the 3 groups of OLPs (including enkephalin and endorphins) was not significant. However, when lymphocytes were incubated with 10~(-7)mol/L MEK, LEK, and β-EP in vitro, naloxone inhibited the strong positive lymphocytes of OLP, and OLP in naloxone and EA groups. Strong positive lymphocyte counts showed significant differences. After incubating lymphocytes with 10~(-12)~10~(-3)mol/L MEK in vitro, the electroacupuncture group showed a concentration of 10~(-10)~10~(-3)mol/L compared with the control group. The interim MEK immune response showed a significant peak, suggesting that electro-acupuncture may increase the activity of potentially unbound MEK receptors.