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共刺激信号是激发有效的细胞免疫应答所必需的.B7分子家族及CD28/CTLA-4等刺激分子在共刺激信号的传递过程中起着重要的作用.T细胞表面CD28与APC细胞表面B7分子结合似乎提供了T细胞激活所需的主要共刺激信号.T细胞表面有多种膜表面分子,这是T细胞识别抗原,与其它免疫细胞相互作用,接受信号刺激等的物质基础,也是鉴别和分离T细胞和T细胞亚群的重要依据.由于共刺激信号的产生和传导,介导靶细胞凋亡可人为调节免疫应答使之增强或抑制.本研究将分析McAb诱导PBLs细胞膜分子表达及肝癌细胞凋亡的相关性.这可能为肿瘤免疫治疗提供新的线索.1 材料与方法1.1 淋巴细胞的分离培养正常人外周血(PBLs)100ml(广州血液中心),2
Co-stimulatory signals are necessary for stimulating effective cellular immune responses. The B7 family and CD28/CTLA-4 stimulatory molecules play an important role in the transmission of costimulatory signals. T cell surface CD28 and APC cell surface B7 molecules The binding seems to provide the major co-stimulatory signal required for T cell activation. There are many membrane surface molecules on the surface of T cells, which is the material basis for T cell recognition antigens, interactions with other immune cells, signal stimulation, etc. Important basis for the isolation of T cells and T cell subsets. As the production and conduction of co-stimulatory signals, mediating apoptosis of target cells can artificially modulate the immune response to enhance or inhibit it. This study will analyze McAb-induced PBLs cell membrane molecule expression and liver cancer Apoptosis correlation. This may provide new clues for tumor immunotherapy. 1 Materials and methods 1.1 Lymphocyte isolation Culture normal human peripheral blood (PBLs) 100ml (Guangzhou Blood Center), 2