目的:建立灯盏花素注射液的细菌内毒素检查法。方法:按《中国药典》2000年版二部收载的细菌内毒素检查方法及指导原则进行实验。将灯盏花素注射液经240倍稀释,用标示灵敏度为0.25EU·ml-1的鲎试剂检测其细菌内毒素。结果:灯盏花素注射液稀释240倍对鲎试剂无干扰作用。结论:可以用细菌内毒素检查法对灯盏花素注射液进行热原检查。 Objective: To establish a bacterial endotoxin test for breviscapine injection. METHODS: Experiments were performed according to the bacterial endotoxin test method and guidelines described in the “Chinese Pharmacopoeia” 2000 edition. Breviscapine injection was diluted by 240-fold and its bacterial endotoxin was detected using a limulus reagent with a sensitivity of 0.25 EU·ml-1. RESULTS: Breviscapine injection was diluted 240 times without interference on limulus reagent. Conclusion: Bacterin endotoxin test can be used for pyrogen test of breviscapine injection.