采用田间试验方法,在苗期和初花期自然状态、水分胁迫以及充足灌水条件下,研究了转DREB3基因抗旱大豆叶片、茎、根中超氧化物歧化酶(SOD)活性,丙二醛(MDA)、脯氨酸和蔗糖含量的变化。结果表明,在自然状态下,苗期水分胁迫第5天时,转DREB3基因抗旱大豆叶片SOD活性显著高于受体大豆东农50,初花期无明显变化;苗期和初花期转DREB3基因抗旱大豆叶片、茎和根中丙二醛、脯氨酸含量与受体大豆东农50相比无明显差异;苗期转DREB3基因抗旱大豆叶片和根中蔗糖含量显著低于受体大豆东农50,初花期差异不显著。在水分胁迫条件下,转DREB3基因抗旱大豆SOD活性显著高于受体大豆东农50,丙二醛含量显著低于受体大豆东农50,初花期水分胁迫第10天时叶片和茎中脯氨酸含量显著高于受体大豆东农50,蔗糖含量显著低于受体大豆东农50。充足灌水管理下,转DREB3基因抗旱大豆与受体大豆东农50相比,苗期和初花期叶片、茎和根中SOD活性、丙二醛和脯氨酸含量无明显差异;苗期转DREB3基因抗旱大豆叶片中蔗糖含量显著低于受体大豆东农50,但在初花期水分胁迫10天时转DREB3基因抗旱大豆叶片中蔗糖含量显著高于受体大豆东农50,苗期和初花期茎和根中蔗糖含量无显著差异。 Field experiments were carried out to study the effects of DREB3 gene on the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in leaves, stems and roots of drought-tolerant soybean at the seedling and early flowering stage under natural conditions, water stress and adequate irrigation. , Proline and sucrose content changes. The results showed that in the natural state, the SOD activity of DREB3 genetically drought-resistant soybean leaves was significantly higher than that of the recipient soybean Dongnong50 on the fifth day after seedling stage water stress, and no significant changes were observed at the first flowering stage. At the seedling and early flowering stage, DREB3 gene drought- The content of malondialdehyde and proline in leaves, stems and roots was not significantly different from that of recipient soybean Dongnong50. The content of sucrose in DREB3-transgenic soybean leaves and roots at seedling stage was significantly lower than that of recipient soybean Dongnong50, Differences in early flowering were not significant. Under water stress, the SOD activity of DREB3 transgenic drought-tolerant soybean was significantly higher than that of the recipient soybean Dongnong50, the content of malondialdehyde was significantly lower than that of the recipient soybean Dongnong50, and the proline The acid content was significantly higher than that of receptor soybean Dongnong50, and the content of sucrose was significantly lower than that of receptor soybean Dongnong50. Under adequate irrigation management, comparing with DREB3 drought-tolerant soybean and recipient soybean Dongnong50, there was no significant difference in SOD activity, malondialdehyde and proline content in leaves, stems and roots at seedling stage and early flowering stage; DREB3 Sucrose content in genetically drought-tolerant soybean leaves was significantly lower than that in recipient soybean Dongnong50, but the content of sucrose in DREB3 transgenic drought-tolerant soybean leaves was significantly higher than that in recipient soybean Dongnong50 at seedling stage of water stress 10 days, and seedling and early flowering stems There was no significant difference in root sucrose content.