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目的研究不同生长期栀子药材中西红花苷和栀子苷的含量与药材清除自由基活性的相关性。方法以前期分离的西红花苷和栀子苷为对照品,采用HPLC测定不同采收期栀子药材中两化合物的含量,并测定药材DPPH自由基的清除活性。结果 HPLC方法准确度和精密度高,方法稳定,线性关系好,西红花总苷含量从8月中旬的0.009%增加到11月中旬的1.32%,而栀子苷则从8月中旬的6.01%降低到12月中旬的2.86%;药材清除自由基的活性变化较小。结论栀子中西红花苷和栀子苷的含量和清除DPPH自由基的能力无相关性。
Objective To study the correlation between the contents of crocin and geniposide in Gardenia officinalis at different growth stages and scavenging free radical activity. Methods The crocin and geniposide isolated from the previous period were used as reference materials. The contents of two compounds in Fructus Gardeniae collected at different harvesting times were determined by HPLC. The scavenging activities of DPPH free radicals were determined. Results HPLC method has the advantages of high accuracy and precision, stable method and good linearity. Total saikoside content increased from 0.009% in mid-August to 1.32% in mid-November, while that of geniposide increased from 6.01 % Decreased to 2.86% in mid-December; herbs to change the activity of free radicals smaller. Conclusion There is no correlation between the content of crocin and geniposide in Gardenia jasminoides and the ability of scavenging DPPH free radical.