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为观察实验性糖尿病大鼠血清一氧化氮与细胞因子的相关性 ,采用链脲佐菌素复制糖尿病动物模型 ,用比色法测定其血清一氧化氮 (nitric oxide,NO)含量和一氧化氮合成酶 (NO synthetase,NOS)活力 ,用放免法测定其血清中肿瘤坏死因子 (tumornecrosis factor,TNFα)和白细胞介素 -6 (Inter leukin-6 ,IL-6 )。结果显示 ,在实验性糖尿病大鼠血清中 NO与 TNFα和 IL -6均呈高度正相关 ,并且随病程延长 NO与 TNFα含量逐渐增加。
To observe the correlation between serum nitric oxide and cytokines in experimental diabetic rats, streptozotocin (STZ) was used to replicate the diabetic animal models, and the levels of serum nitric oxide (NO) and nitric oxide The activity of NO synthetase (NOS) was measured by radioimmunoassay. Serum tumor necrosis factor (TNF) and interleukin-6 (IL-6) levels were measured by radioimmunoassay. The results showed that there was a highly positive correlation between NO and TNFα and IL-6 in the serum of experimental diabetic rats, and the content of NO and TNFα gradually increased with the course of disease.