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目的:探讨成纤维细胞生长因子-10(FGF-10)促创面肉芽组织形成的作用机制。方法:将不同浓度的 FGF-10分别加入细胞培养液中,于作用后24、48和7,2h 收集细胞培养上清,采用 ELISA 方法测定粒细胞—巨噬细胞集落刺激因子 GM-CSF 的含量,同时进行细胞计数。结果:当细胞接种密度为2 500细胞/cm~2时,24h 的培养上清中未能检测到 GM-CSF,48h 的上清中125 ng/ml 和500ng/ml FGF-10组 GM-CSF 的浓度及单个细胞的 GM-CSF 的分泌均显著高于对照组(P<0.05)。72h的培养上清中仅500ng/ml FGF-10组 GM-CSF 的分泌量显著高于对照组(P<0.05)。当细胞接种密度为5 000细胞/cm~2时,16～500ng/ml 的 FGF-10各组 GM-CSF 浓度显著高于对照组(P<0.05),但单个细胞的 GM-CSF 分泌量与对照组无显著差异(P>0.05),48h 收集的培养上清中,与对照组相比,FGF-10各组的 GM-CSF 均未升高,且48h 各组单个细胞的GM-CSF 分泌量与该培养皿中的细胞总数呈负相关(r=-0.881,P<0.05)。结论:实验结果提示 FGF-10可能通过刺激 GM-CSF 的分泌,从而间接作用于成纤维细胞、内皮细胞等,促进创面肉芽组织形成。
Objective: To investigate the mechanism of granulation tissue formation induced by fibroblast growth factor-10 (FGF-10). Methods: Different concentrations of FGF-10 were added to the cell culture medium, and the cell culture supernatants were collected at 24, 48, 7 and 2 hours after treatment. The concentration of granulocyte-macrophage colony stimulating factor GM-CSF was measured by ELISA , While the cell count. Results: GM-CSF could not be detected in 24h culture supernatant when the cell inoculation density was 2 500 cells / cm ~ 2, while in the supernatant of 48h, 125 ng / ml and 500 ng / ml FGF-10 GM-CSF (P <0.05), and the secretion of GM-CSF in single cells were significantly higher than those in control group (P <0.05). The secretion of GM-CSF in 500ng / ml FGF-10 group was significantly higher than that in control group (P <0.05) in 72h culture supernatant. When the cell inoculation density was 5000 cells / cm2, the concentration of GM-CSF in 16 ~ 500ng / ml FGF-10 group was significantly higher than that in control group (P <0.05) There was no significant difference in the control group (P> 0.05). Compared with the control group, the GM-CSF of the FGF-10 groups did not increase in the 48h culture supernatant, and the secretion of GM-CSF The amount was negatively correlated with the total number of cells in the Petri dish (r = -0.881, P <0.05). Conclusion: The results suggest that FGF-10 may stimulate the secretion of GM-CSF and indirectly act on fibroblasts and endothelial cells to promote wound granulation tissue formation.