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目的 明确Ⅰ型人免疫缺陷病毒 (humanimmunodeficiencyvirustype 1,HIV 1)包膜糖蛋白gp12 0第三可变区 (variableregion ,V3)在病毒进入靶细胞的早期阶段中的作用。方法 合成了 3个环状V3多肽 ,包括V3 BH10、V3 ADA和V3 89.6以及直链和短链多肽V3 BH10 /CA、V3 NNT2 4和V3 IRI12。构建了 3株分别带HIVHXB2株、ADA株和 89.6株包膜的伪病毒并观察了多肽对不同嗜性HIV侵入靶细胞能力的影响。结果 (1)HIV 1V3区多肽以毒株特异性模式增强HXB2、ADA和 89.6进入靶细胞 ;(2 )直链V3多肽与其环状多肽具有相似作用 ;带有C末端的短肽V3 NNT2 4也有与长链V3 BH10 /CA相近的作用 ,只有中央保守区的短链V3 IRI12没有类似功能。结论 本研究首次发现HIV 1V3区在病毒进入靶细胞早期阶段中具有毒株特异性增强病毒侵入的作用。该发现有助于进一步明确HIV 1进入靶细胞的机制
Objective To clarify the role of the variable region (V3) of the human immunodeficiency virus 1 (HIV 1) envelope glycoprotein gp12 0 in the early stages of virus entry into target cells. Methods Three cyclic V3 polypeptides were synthesized, including V3 BH10, V3 ADA and V3 89.6, and the linear and short chain polypeptides V3 BH10 / CA, V3 NNT2 4 and V3 IRI12. Three strains of pseudovirus containing HIVHXB2, ADA and 89.6 strains were constructed and their effects on the ability of different tropic HIV invade target cells were observed. Results (1) The HIV 1V3 polypeptide enhanced HXB2, ADA and 89.6 into the target cells in a strain-specific pattern. (2) The linear V3 peptide had a similar effect with its cyclic polypeptide. The short peptide with C terminal, V3NNT2 4, also had Similar to the long-chain V3 BH10 / CA, only the short-chain V3 IRI12 in the central conserved region has no similar function. Conclusions This study, for the first time, found that the HIV 1V3 region has a strain-specific enhancement of viral entry during the early stages of virus entry into target cells. This finding helps to further elucidate the mechanism by which HIV 1 enters target cells