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用0-0.20 kGy钴-60γ射线对含华支睾吸虫囊迹的鱼体和从鱼体中分离所得的囊蚴(离体囊蚴)分别进行照射,观察照射囊蚴在宿主(动物)体内发育的能力。囊蚴以灌胃法感染豚鼠或大鼠。用粪检虫卵和尸剖动物检查虫体的生物学方法,判定控制华支睾吸虫囊蚴感染性的最小有效剂量。结果表明,照射离体囊蚴的最小有效剂量为0.05kGy。照射鱼体组织内囊蚴的半数致死量为0.05kGy,最小有效剂量为0.15kGy。我国北方、中部和南方的该虫株囊蚴对钴-60γ射线的敏感性无显著差异,认为用钻-60γ射线0.15kGy剂量可完全控制鱼体中华支睾吸虫囊蚴的感染性,因而辐照技术可作为预防医学中的一种方法。
Radiograms of Clonorchis sinensis Clostridium and the metacercariae isolated from the fish were irradiated with 0-0.20 kGy Cobalt-60γ ray respectively to observe the effect of irradiation on the host (animal) Developmental ability. Cysticercosis by gavage infected guinea pigs or rats. Biological methods for examining parasites using fecal insecticidal eggs and carcasses were used to determine the minimum effective dose to control Clonorchis sinensis metacercariae infectivity. The results showed that the minimum effective dose of irradiation of metacercariae 0.05kGy. The median lethal dose of metacercariae in the irradiated tissue was 0.05 kGy and the minimum effective dose was 0.15 kGy. In northern China, central China and southern China, the worm metacercariae had no significant difference in the sensitivity to cobalt-60γ rays. It was concluded that the 0.15kGy dose of rhG-60γ ray could completely control the infectivity of the metacercariae of Clonorchis sinensis in fish, According to technology can be used as a preventive medicine in a way.