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应用微孔滤膜法研究巨噬细胞在单核细胞迁移过程中所起的作用 ,结果表明 ,兔腹腔巨噬细胞能够产生单核细胞趋化因子。该因子具耐热性 ,加热至 10 0℃ 10 min仍有活性 ;经透析其活性仍然保存 ;但可被胰蛋白酶灭活 ;对巯基乙醇敏感。提示该因子是一种含关键二硫键的多肽
The role of macrophages during monocyte migration was studied by microporous membrane filtration. The results showed that monocytes were produced by monocytes peritoneal macrophages. The factor has heat resistance, is still active after heated to 10 0 ℃ 10 min; its activity is still preserved by dialysis; but can be inactivated by trypsin; sensitive to mercaptoethanol. Suggesting that the factor is a key disulfide-containing polypeptide