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本研究利用mtDNACOI基因的部分序列对11种群不同寄主的栗瘿蜂Dryocosm us kuriphilus Yasumatsu遗传多态性进行了分析。在获得的660bp的序列中,变异位点(V)80个,包括简约信息位点(Pi)15个和自裔位点(Si)65个。T、A、C、G平均含量分别为30.6%、43.3%、13.7%和12.4%,A+T平均值为73.9%,远高于G+C的26.1%,表现出明显的A+T偏向性。为害锥栗的六步溪、大围山种群与茅栗、板栗的不同地理种群,以及为害欧洲栗的意大利Piedmont种群之间,基因序列完全一致或者遗传距离在0.3%以内,无论是NJ系统树还是UPGMA系统树均聚合在同一分支内,亲缘关系较近。而为害锥栗的采自福建省建瓯市水源镇的水源种群、福建省周宁县纯池镇的纯池种群,与其它各栗瘿蜂种群的基因序列差异较大,遗传距离均大于2%,在NJ和UPGMA系统树中水源种群和纯池种群均聚合为一单独的分支,与其它种群形成的分支互为姊妹分支。据此我们认为水源种群和纯池种群很有可能属于不同的物种,有进一步研究的必要。
In this study, genetic diversity of Dryocosmus kuriphilus Yasumatsu in 11 populations of different hosts was analyzed by partial sequence of mtDNACOI gene. Among the 660bp sequences obtained, there were 80 mutated sites (V), including 15 parsimony informative sites (Pi) and 65 self-locus (Si) sites. The average contents of A, T, A, C and G were 30.6%, 43.3%, 13.7% and 12.4%, respectively. The A + T average was 73.9%, much higher than 26.1% of G + C, showing obvious A + T bias Sex. The genetic sequences of six-step stream, Daweishan population and the different geographical populations of chestnut and chestnut, as well as the Piedmont population of European chestnut, were identical or less than 0.3% Or UPGMA system tree are aggregated in the same branch, the kinship closer. The damage of Castanea henryi collected from water source town of Jian’ou City, Fujian Province, the pure pool of Chunchi Town, Zhouning County, Fujian Province, showed great difference in genetic sequence with other populations of Castanopsis kawakamii, the genetic distance was more than 2% In the NJ and UPGMA phylogenetic tree, the water source population and pure pool population all converge into a single branch, which is a sister branch to the other populations. Based on this, we think that the water source population and the pure-pool population are likely to belong to different species, which is necessary for further research.