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采用超声提取法对罗布麻叶中总生物碱进行了提取,用紫外可见光谱法进行了测定。确定了提取总生物碱所需提取溶剂的适当浓度。建立了苦参碱浓度与吸光度关系的回归方程:C=0.0449A-0.0068,r=0.9911。该方法简单、提取率高、重现性好。
The total alkaloids in Apocynum venetum L. leaves were extracted by ultrasonic extraction and determined by UV-visible spectroscopy. The appropriate concentration of extraction solvent required to extract the total alkaloids was determined. The regression equation of matrine concentration and absorbance was established: C = 0.0449A-0.0068, r = 0.9911. The method is simple, high extraction rate, good reproducibility.