论文部分内容阅读
从乳猪脑中提取相应的神经营养物质,应用胎鼠大脑皮层细胞培养技术和四唑盐(M-TT)微量比色法,观察了乳猪脑提取物和脑活素(Cerebrolysin)对大脑皮层细胞培养的影响。结果表明,乳猪脑提取物能起明显促进神经元突起生长和支持存活的作用,并存在着剂量作用效应,当提取物剂量为20mg/L时作用效果最佳。脑活素对皮层神经元促突起生长和支持存活的作用不理想。MTT微量比色法结果显示,乳猪脑组织提取物能明显增强皮层神经元内线粒体脱氢酶的活力,并存在剂量相关效应。以上结果表明,乳猪脑提取物中含有神经营养因子(Neurotrophic Factors, NTFs)成分,并非由于氨基酸或多肽的单纯营养作用所致。
The corresponding neurotrophic substances were extracted from the brain of suckling pigs. Cerebrospinal fluid (FCM) and fetal bovine cerebral cortex cell (MTT) microtitration method were used to observe the effects of cerebrum extract and cerebrolysin on brain Effect of cortical cell culture. The results showed that LBP could significantly promote neurite outgrowth and support survival, and there was dose effect. When the extract dosage was 20mg / L, the effect was the best. Cerebrolysin is not ideal for promoting neurite outgrowth and supporting survival in cortical neurons. The results of MTT microdialysis showed that extracts from brain of piglets significantly increased mitochondrial dehydrogenase activity in cortical neurons and had dose-related effects. The above results show that milk brain extracts contain neurotrophic factors (Neurotrophic Factors, NTFs) components, not due to simple amino acid or polypeptide nutrition.