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目的观察树莓酮对非酒精性脂肪性肝炎(NASH)大鼠血液中肿瘤坏死因子(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-18(IL-18)的影响。方法 SD大鼠50只,分为5组:正常对照组(NC),模型组(M)树莓酮低剂量组(RKL)树莓酮中剂量组(RKM)和树莓酮高剂量组(RKH)。采用高脂饲料喂养复制NASH大鼠模型。在RKL组、RKM组和RKH组饲喂高脂饲料4周后,分别以浓度为5g/L,10g/L,20g/L的树莓酮溶液进行干预。给药4周后,处死大鼠,采集肝脏标本,HE染色作肝脏病理组织学检查;醋酸双氧铀柠檬酸铅染色,透射电镜观察肝脏组织超微结构变化;酶联免疫法(ELISA)测量血液中TNF-α、IL-1和IL-18的水平。结果与NC组比较,M组肝细胞出现明显脂肪变性,肿大,胞浆内充满脂肪空泡或脂滴,线粒体嵴消失等结构异常改变明显并且血液中TNF-α、IL-1和IL-18的水平显著升高(P<0.05)。与M组比较,RKL、RKM、RKH三个剂量组肝细胞异常结构均有不同程度的改善并且三个剂量组血液中的TNF-α、IL-1和IL-18水平均有不同程度降低(P<0.05)。结论树酶酮改善NASH的作用,可能与降低大鼠血液中炎症因子TNF-α、IL-1和IL-18的水平相关。
Objective To observe the effects of raspberry ketone on the levels of tumor necrosis factor (TNF-α), interleukin-1 (IL-1), interleukin-18 (IL-18) in nonalcoholic steatohepatitis (NASH) influences. Methods Fifty SD rats were divided into five groups: normal control group (NC), model group (M) raspberry ketone low dose group (RKL) raspberry ketone medium dose group (RKM) and raspberry ketone high dose group RKH). Fed with high fat diet to replicate NASH rat model. Four weeks after fed with high-fat diet, RKL group, RKM group and RKH group were treated with raspberry ketone solution of 5g / L, 10g / L and 20g / L, respectively. Four weeks after administration, the rats were sacrificed and the liver specimens were harvested for histopathological examination of liver. Uranyl acetate was stained with lead citrate and the ultrastructural changes of liver tissues were observed by transmission electron microscopy. Enzyme-linked immunosorbent assay (ELISA) The levels of TNF-α, IL-1 and IL-18 in the blood. Results Compared with NC group, there were obvious steatosis and enlargement of hepatocytes in M group, obvious changes of vacuoles and lipid droplets in the cytoplasm, disappearance of mitochondrial cristae and other structural abnormalities. The levels of TNF-α, IL-1 and IL- 18 levels were significantly increased (P <0.05). Compared with M group, the abnormal structure of hepatocytes in RKL, RKM and RKH groups improved to some extent and the levels of TNF-α, IL-1 and IL-18 in the three dose groups were all decreased to some extent P <0.05). Conclusions Enalase can improve the function of NASH, which may be related to the decrease of the levels of inflammatory cytokines TNF-α, IL-1 and IL-18 in rats.