论文部分内容阅读
目的研究白花丹醌对人肝星状细胞株(HSC-LX2)的细胞毒性作用,并通过细胞周期及凋亡率的变化探讨该药抑制细胞增殖的机制。方法 HSC-LX2与药物孵育48h,以MTT法检测细胞毒性;以流式细胞术PI染色法检测各周期细胞DNA的含量及细胞晚期凋亡率。结果白花丹醌低、中、高浓度对HSC-LX2细胞均具有明显的细胞毒性并抑制其细胞增殖;G_0/G_1期细胞的比例发生升高而S期+G_2/M期细胞总数降低;且细胞的晚期凋亡率明显增高。以上作用具有剂量依赖性,有统计学差异(P<0.05)。结论白花丹醌对HSC-LX2具有明显的细胞毒性而对肝纤维化进程有干预作用,原因为将细胞周期阻滞在G_0/G_1期而阻止细胞的分裂增殖,并能诱导细胞发生凋亡。上述能力均有剂量依赖性;且中、高剂量白花丹醌的干预能力强于秋水仙碱和复方鳖甲软肝片。
Objective To study the cytotoxicity of plumbagin on human hepatic stellate cell line (HSC-LX2) and to explore the mechanism of its inhibition on cell proliferation by the changes of cell cycle and apoptosis rate. Methods HSC-LX2 was incubated with drugs for 48 hours and the cytotoxicity was detected by MTT assay. The content of DNA and the late apoptosis rate of cells in each cycle were detected by flow cytometry (PI) staining. Results Low, medium and high concentrations of plumbagin had obvious cytotoxicity and inhibited the proliferation of HSC-LX2 cells. The proportion of cells in G 0 / G 1 phase increased and the total number of cells in S phase and G 2 / M phase decreased. Cell apoptosis rate was significantly higher. The above effects were dose-dependent, with statistical significance (P <0.05). Conclusions Plumbagin has obvious cytotoxicity on HSC-LX2 and may interfere with the process of hepatic fibrosis. The reason is that blocking the cell cycle in G_0 / G_1 phase can prevent cell proliferation and induce cell apoptosis. The above-mentioned abilities are dose-dependent; and middle-dose and high-dose plumbagin have better intervention ability than colchicine and Fufang Biejia Ruangan Tablet.