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本文利用柱层析法从天然产物中提取分离了叶绿素a(Chl-a)和胡罗h素C(Car),并按Langmuir-Blodgett单分子膜技术制备了Chl-a单分子膜、Chl-a与Car的混合单分子膜。实验中还引入了硬脂酸起辅助支撑作用,选用的硬脂酸与Chl-a的摩尔比为10:1。挂膜载片为经过疏水处理的玻璃片。为制作模仿天然光合膜集光天线系统的功能,我们以Car为给体Chl-a为受体,Caf与Chl-a的摩尔比为5:1、50:1和125:1;这里选用的比例远大于集光天线的色素比例。用荧光光谱的测量发现,L-B膜内的色素分子有特殊的集聚态,这种状态有助于实现高效的激子转移。作者指出,由于二维的L-B膜上会发生Frster型和激子型两种能量转移的协同作用,所以在Car与Chl-a的摩尔比125:1时Chl-a的荧光无相应衰减。Chl-a单分子膜的荧光带为677nm和766nm。吸附在层析纸上Chl-a的荧光,仅长波带有明显位移。
In this paper, Chl-a and Carotenoid C were extracted from natural products by column chromatography and Chl-a monolayers were prepared according to the Langmuir-Blodgett monolayer technique. Chl- a mixed with Mono Mon film. In the experiment, stearic acid was also introduced as an auxiliary support. The molar ratio of stearic acid to Chl-a was 10: 1. Sliding film slides are hydrophobic treated glass slides. In order to imitate the function of natural photosynthetic membrane collecting antenna system, we use Car as donor Chl-a as acceptor, the molar ratio of Caf to Chl-a is 5: 1, 50: 1 and 125: 1; The proportion is much larger than the pigment ratio of the collecting antenna. Fluorescence spectroscopy measurements showed that the L-B membrane pigment molecules have a special aggregation state, this state helps to achieve efficient exciton transfer. The authors note that there is no corresponding attenuation of the fluorescence of Chl-a at a Car: Chl-a molar ratio of 125: 1 due to the synergistic effect of the two energy shifts of the Frster and exciton modes on the two-dimensional L-B membrane. The fluorescent bands of Chl-a monolayers were 677 nm and 766 nm. Fluorescence of Chl-a adsorbed on the chromatographic paper, with only significant shift in the longwave.