目的:建立以高效液相色谱法测定经舒胶囊中丹皮酚含量的方法。方法:采用Platisil ODS色谱柱(4.6 mm×250 mm 5μm);以甲醇-水(45:55)为流动相;柱温:40℃;流速:1.0ml.min-1;检测波长:274nm。结果:在建立的色谱条件下,丹皮酚进样量在0.08487~0.50922μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为98.75%(RSD=1.74%)。结论:方法简便可靠,分离度好,可用于经舒胶囊的质量控制。 Objective: To establish a method for the determination of paeonol in Jingshu capsules by high performance liquid chromatography. Methods: Platisil ODS column (4.6 mm × 250 mm 5 μm) was used. The mobile phase consisted of methanol and water (45:55). The column temperature was 40 ℃. The flow rate was 1.0 ml · min-1. The detection wavelength was 274 nm. Results: Under the established chromatographic conditions, the paeonol injection showed a good linear relationship with the peak area in the range of 0.08487 ~ 0.50922μg (r = 0.9999) and the average recovery was 98.75% (n = 6) (RSD = 1.74%). Conclusion: The method is simple and reliable with good separation and can be used for the quality control of Jingshu capsule.