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以南农1138-2为材料,采用不同浓度(0,0.20,0.40,0.60和0.80 mmol·L-1)的H2S供体Na HS溶液喷施在0.08 mol·L-1的Na Cl溶液胁迫下的大豆植株上,分析大豆从V2至V4期生长和同工酶活性的影响。结果表明:0.08mol·L-1的Na Cl溶液能有效抑制大豆生长。H2S的供体Na HS溶液喷施对盐胁迫有一定的缓解作用,0.20和0.40mmol·L-1的Na HS处理的子叶等级显著高于阳性对照(0.00 mmol·L-1Na HS处理),0.40 mmol·L-1的Na HS处理有最大的根冠比。0.40 mmol·L-1的Na HS处理下的V3和V4期大豆叶片中的过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性虽然比阴性对照低,但比其余处理都高,并都显著高于阳性对照。并且在V3期大豆的子叶等级与抗氧化酶活性成正相关关系。POD和SOD的同工酶在Na HS处理下谱带都发生了一定程度的改变。
Using Nannong 1138-2 as the material, NaHS solution with different concentrations (0,0.20,0.40,0.60 and 0.80 mmol·L-1) was sprayed under NaCl stress of 0.08 mol·L-1 Of soybean plants, the effects of soybean growth and isoenzyme activity from V2 to V4 phases were analyzed. The results showed that: 0.08mol·L-1 NaCl solution can effectively inhibit soybean growth. H2S donor Na HS solution sprayed on salt stress have some relief, 0.20 and 0.40mmol · L-1 of Na HS treatment cotyledonary grade was significantly higher than the positive control (0.00 mmol · L-1Na HS treatment), 0.40 Na HS treatment with mmol·L-1 had the highest root-shoot ratio. The activity of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in V3 and V4 soybean leaves treated with 0.40 mmol·L-1 NaHS was lower than that of negative control Low, but higher than the rest of the treatment, and were significantly higher than the positive control. And in the V3 soybean cotyledon level and antioxidant enzyme activity has a positive correlation. The isozymes of POD and SOD changed to some extent under Na HS treatment.