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本所采用MCDB151,并补充多种生长因子的无血清培养基,用组织块培养或酶消化的方法,已成功培养18例成人支气管上皮细胞。这些上皮细胞一般可连续传代5-6次(与国外报道一致).其中培养时间最长的组织块已有7个多月(已移接13次),并能继续长出上皮细胞,这株上皮细胞目前已传至第9代。用这种无血清培养基(或再加入少量血清)已培养4例肺癌组织(腺癌和鳞癌各2例),均可长出上皮细胞,
The Institute used MCDB151, supplemented with a variety of growth factor-free serum-free medium, and successfully cultured 18 adult human bronchial epithelial cells by tissue culture or enzyme digestion. These epithelial cells can generally be serially passaged 5-6 times (consistent with foreign reports). The longest cultured tissue block has been more than 7 months (13 times has been transferred), and can continue to grow epithelial cells, this strain Epithelial cells have now been transmitted to the 9th generation. Using this serum-free medium (or adding a small amount of serum), 4 lung cancer tissues (adenocarcinoma and squamous cell carcinoma, 2 cases each) have been cultured to grow epithelial cells.