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目的:观察糖尿病小鼠周围神经病变(DPN)发生过程中感觉神经功能的变化过程,评价甲钴胺、格列齐特及其联合用药对链脲佐菌素糖尿病(STZ-D)小鼠周围神经病变的防治效果。方法:小鼠进行STZ造模后,连续灌胃给药12周,动态测定STZ-D小鼠对热、冷刺激反应时间、负重攀网时间以及化学刺激疼痛反应舔足次数,并对药物的治疗效果进行观察。结果:STZ造模后2周时,STZ-D小鼠热刺激反应和化学刺激的疼痛反应明显性增强,4至8周后则又明显性减弱,感觉神经功能表现出明显的双向性变化;STZ-D小鼠对冷刺激反应也显示出相似的双向性变化或变化趋势;STZ-D小鼠的负重攀网时间不但较正常动物有明显性降低,而且同时也存在着先明显性的降低、再部分恢复,然后又明显降低的双向变化过程。结论:甲钴胺与格列齐特及其联合用药对以上变化均表现出一定的治疗效果;除负重攀网时间外,甲钴胺与格列齐特联合应用未表现出明显的作用增强效果。本实验结果对DPN的研究和药物治疗可提供参考。
OBJECTIVE: To observe the changes of sensory nerve function during the development of peripheral neuropathy (DPN) in diabetic mice and to evaluate the effects of mecobalamin, gliclazide and their combination on the peripheral blood of streptozotocin-diabetic (STZ-D) mice Prevention and treatment of neuropathy. Methods: After STZ model was established, the rats were administrated with gavage for 12 weeks. The dynamic response of STZ-D mice to heat and cold stimulation, Therapeutic effect was observed. Results: After 2 weeks of STZ-induced STZ-D mice, the pain response of thermal stimulation and chemical stimulation significantly increased, and significantly decreased after 4 to 8 weeks. Sensory nerve function showed obvious bidirectional changes. STZ-D mice also showed similar bidirectional changes or trends in cold stimulation response. The load-climbing time of STZ-D mice was not only significantly lower than that of normal animals, but also significantly decreased , And then partially restored, and then significantly reduce the two-way change process. CONCLUSION: Both mecobalamin and gliclazide and their combination therapy showed some therapeutic effects on the above changes. In addition to the time of loading, the combination of mecobalamin and gliclazide did not show any significant effect . The experimental results on the DPN research and drug treatment can provide a reference.