嵌合病毒技术是用于RNA病毒研究的一个重要技术,近十年来在黄热病毒属病毒的研究中,构建了一些型内及型间的嵌合病毒,不仅有利于该属病毒的分子机制研究,更重要的是研制出一些新型减毒活疫苗。本文简单介绍该技术的原理、构建、影响因素、应用前景等。 Chimeric virus technology is an important technology used in the research of RNA viruses. In the research of the yellow fever virus in the recent ten years, a number of intra-and inter-type chimeric viruses have been constructed, which is not only beneficial to the molecular mechanism of the virus Research, more importantly, to develop some new live attenuated vaccine. This article briefly introduces the principle of the technology, construction, influencing factors, application prospects and so on.