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目的为探寻灰黄霉素生物合成过程中的关键酶,以蛋白质组学技术手段分析灰黄霉素高产菌F208发酵过程蛋白质表达差异。方法通过双向电泳联用质谱技术对F208发酵过程蛋白质组图谱进行比较分析。结果研究发现灰黄霉素产生期(192 h)F208表达的蛋白质与产生前期(72 h)有较大差异,并鉴定出在灰黄霉素产生高峰期蛋白表达量明显增加的两个特异点是丝氨酸羟甲基转移酶和S-腺苷甲硫氨酸合成酶。结论成功建立灰黄青霉菌丝体总蛋白双向电泳技术体系。并鉴定出两个蛋白特异点,极可能与灰黄霉素生物合成有关。
Aim To explore the key enzyme in the process of griseofulvin biosynthesis, proteomic analysis of proteoglycan of F208 with high-yielding griseofulvin was analyzed by proteomic techniques. Methods Two-dimensional electrophoresis with mass spectrometry was used to compare the proteome profiles of F208 fermentation. Results The results showed that the expression of F208 in the griseofulvin production period (192 h) was significantly different from that in the pre-production stage (72 h), and two specific spots were identified in which peak expression of griseofulvin was significantly increased Is serine hydroxymethyltransferase and S-adenosylmethionine synthase. Conclusion The two-dimensional electrophoresis system of total protein of Penicillium chrysogenum was successfully established. And identified two specific protein spots, most likely related to griseofulvin biosynthesis.