论文部分内容阅读
以40份西南地区玉米地方品种群体为材料,对Waxy基因第9外显子和第14外显子之间的DNA序列进行测序,将测序得到的40条序列与Gen Bank中来自普通玉米、小颖玉米、墨西哥玉米和二倍体多年生玉米的20条同源序列作比对分析。结果表明,来自西南地区四川、重庆、云南和贵州的玉米地方品种群体分别有180、152、163、164个多态性位点,供试种群遗传多态性指数依次为小颖玉米>四川地方品种>墨西哥玉米>重庆地方品种>二倍体多年生玉米>贵州地方品种>云南地方品种>普通玉米。Tajima’D、Fu and Li’D和Fu and Li’F检验表明,Waxy基因是中性进化基因。比较种群间Waxy基因序列的共有多态性和固有差异,玉米地方品种与墨西哥玉米和二倍体多年生玉米间存在较高的固有差异和较低的共有多态性。Waxy基因的系统演化分析结果表明,小颖玉米、普通玉米和玉米地方品种有相近的遗传关系,小颖玉米是普通玉米和玉米地方品种的共同祖先。
The DNA sequences of exon 9 and exon 14 of Waxy gene were sequenced from 40 populations of maize landraces in Southwest China. The 40 sequences were sequenced and compared with those from GenBank Comparative analysis of 20 homologous sequences of Ying corn, Mexican corn and diploid perennial corn. The results showed that there were 180, 152, 163 and 164 polymorphic loci in maize from Sichuan, Chongqing, Yunnan and Guizhou in Southwest China. The genetic polymorphism index of the tested populations were in order of Xiaoying corn> Sichuan province Varieties> Mexican corn> Chongqing local breeds> Diploid perennial maize> Guizhou local breeds> Yunnan local breeds> ordinary corn. Tajima’D, Fu and Li’D and Fu and Li’F tests showed that the Waxy gene is a neutral evolutionary gene. Comparing the common polymorphisms and inherent differences of Waxy gene sequences among populations, there are high inherent differences and low common polymorphisms between maize landraces and Mexican corn and diploid perennial maize. The phylogenetic analysis of Waxy gene showed that there was a close genetic relationship between Xiaoyan maize, common maize and maize local breeds. Xiaoying maize was the common ancestor of common maize and maize local breeds.