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目的构建恶性疟原虫海南分离株（FCC1／HN）裂殖子表面蛋白MSA2的真核表达质粒pcDNA3／MSA2，为疟疾核酸疫苗及蛋白疫苗的研制奠定基础。方法采用PCR技术对FCC1／HN基因组DNAMSA2基因进行扩增，扩增产物经纯化后用BamHI和EcoRI双酶切，然后走向克隆入真核表达质粒pcDNA3，连接产物转化大肠杆菌TG1，再用相同的内切酸酶切和PCR扩增对重组子进行鉴定。结果筛选出的重组子为编码PCC1／HCMSA2基因片段的重组质粒pcDNA3／MSA2。结论编码FCC1／HNMSA2基因片段真该表达质粒pcDNA3／MSA2的构建，为疟疾核酸疫苗及蛋白疫苗的研制奠定基础。
Objective To construct eukaryotic expression plasmid pcDNA3 / MSA2 of merozoite surface protein MSA2 of Plasmodium falciparum Hainan (FCC1 / HN), and lay a foundation for the development of malaria nucleic acid vaccine and protein vaccine. Methods The MSA2 gene of FCC1 / HN genomic DNA was amplified by PCR. The amplified product was digested with BamHI and EcoRI and then cloned into the eukaryotic expression plasmid pcDNA3. The ligated product was transformed into E. coli TG1. The same Recombinant was identified by endonuclease digestion and PCR amplification. Results The recombinants screened were pcDNA3 / MSA2, a recombinant plasmid encoding PCC1 / HCMSA2 gene. Conclusion The recombinant plasmid pcDNA3 / MSA2 encoding FCC1 / HNMSA2 gene was constructed and laid the foundation for the development of malaria nucleic acid vaccine and protein vaccine.