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选取山茱萸多糖提取时间、提取温度和料液比3个因素进行二次回归正交组合设计试验,利用响应面法对其提取工艺参数进行优化研究。利用MATLAB 7.0软件对山茱萸多糖提取产量的二次多项数学模型解逆矩阵分析表明:在提取时间为142 min、提取温度为84℃、水体积为276 mL的条件下,山茱萸多糖提取产量最高,最大提取产量预测值为89.7868μg·mL~(-1),实际提取值89.34μg·mL~(-1),两者基本相符。利用优化工艺参数提取山茱萸多糖时,具有最大的提取产量。
The quadratic regression orthogonal design experiment was carried out with the extraction time, extraction temperature and material-liquid ratio of polysaccharide from Hawthorn, and the extraction process parameters were optimized by response surface method. The use of MATLAB 7.0 software for inverse quadratic polynomial model analysis of extraction yield of hawthorn polysaccharides showed that Hawthorn polysaccharides had the highest extraction yield at the extraction time of 142 min, extraction temperature of 84°C, and water volume of 276 mL. The predicted maximum extraction yield was 89.7868 μg·mL -1 and the actual extracted value was 89.34 μg·mL -1 , which were basically consistent. Extraction of hawthorn polysaccharides with optimized process parameters has the greatest extraction yield.