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目的：克隆α粒子辐射致细胞恶性转化相关基因。方法：采用ｍ Ｒ Ｎ Ａ 差异显示技术识别原代大鼠气管上皮细胞同由α粒子辐射诱发恶转的大鼠气管上皮细胞之间的差别表达基因。结果：共克隆到１５ 个可能同α粒子辐射致细胞恶性转化相关的差异表达基因片段（ Ｅ Ｓ Ｔｓ） ，共向 Ｇｅｎ Ｂａｎｋ 登录７ 条新基因序列。其中克隆 Ｚ Ｃ６６ 同肿瘤转移抑制基因ｎｍ ２３ 高度同源，经 Ｎｏｒｔｈｅｒｍ 杂交证实其在恶转的大鼠气管上皮细胞中高表达，序列分析提示ｎ ｍ２３在此恶转细胞中存在突变。结论：上述结果为进一步深入研究辐射致癌提供了线索，并提示ｎ ｍ２３ 基因可能参与α粒子辐射致大鼠气管上皮细胞恶性转化的原发过程。
OBJECTIVE: To clone the genes involved in the malignant transformation of cells induced by alpha particles. METHODS: Differentially expressed genes between primary rat tracheal epithelial cells and rat tracheal epithelial cells induced by alpha particle irradiation were identified using the m R N A differential display technique. RESULTS: A total of 15 differentially expressed gene fragments (ESTs) that might be involved in the malignant transformation of α-irradiation cells were cloned and seven new gene sequences were registered with Gen Bank. Among them, clone Z C66 is highly homologous to tumor metastasis suppressor gene nm23. It was confirmed by Northerm hybridization that it was highly expressed in dysplastic rat tracheal epithelial cells. Sequence analysis revealed that n m23 has mutations in this malignant transduced cell. Conclusion: These results provide clues for further investigation of radiation carcinogenesis and suggest that nm23 gene may be involved in the primary process of malignant transformation of rat tracheal epithelial cells induced by alpha radiation.