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目的:通过放射免疫方法观测胃溃疡模型大鼠胃黏膜中表皮生长因子(EGF)和前列腺素E2(PGE2)含量,分析健脾化浊方提高胃溃疡愈合质量可能的作用机制。方法:参照Okabe的方法并加以改进造模,成功后将其随机分为空白对照组、健脾化浊方组和雷尼替丁组,每组各8只,另设正常对照组8只,连续灌胃14天后取大鼠胃黏膜溃疡灶或溃疡瘢痕及其周围组织,用放射免疫法观测组织中EGF和PGE2含量。结果:健脾化浊方可提高胃溃疡模型大鼠胃黏膜中EGF、PGE2含量,此可能为提高胃溃疡愈合质量的机制之一。
OBJECTIVE: To observe the content of epidermal growth factor (EGF) and prostaglandin E2 (PGE2) in gastric mucosa of gastric ulcer model rats by radioimmunoassay, and to analyze the possible mechanism of Jianpi Huazhuo decoction in improving the healing quality of gastric ulcer. Methods: Refer to Okabe’s method and improve the model. After success, they were randomly divided into blank control group, Jianpi Huazhuo group and ranitidine group, 8 in each group, and 8 normal control groups. After 14 consecutive days of gastric perfusion, gastric mucosal ulcer lesions or ulcer scars and their surrounding tissues were taken, and the contents of EGF and PGE2 in the tissues were observed by radioimmunoassay. Results: Jianpi Huazhuo Prescription could increase the content of EGF and PGE2 in gastric mucosa of gastric ulcer model rats, which may be one of the mechanisms of improving the healing quality of gastric ulcer.