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目的:观察糖尿病是否与唾液中氧化还原动态平衡的改变相关。方法:用ELISA方法分析148名糖尿病患者和475名对照的非刺激性唾液中氧胁迫损伤标志物8-羟基-2′-脱氧鸟苷(8-hydroxy-2’-deoxyguanosine,8-OHdG)、蛋白质羰基(Protein carbonyls,PC)和8-异前列腺素F2α(8-epi-prostaglandin-F2α,8-epi-PGF2α)的含量。用逻辑相关分析的统计学方法分析氧胁迫损伤指标与使用胰岛素、二甲双胍和餐饮控制糖尿病患者之间的相关性。结果:(i)与对照组相比糖尿病组唾液中的Log8-OHdG、LogPC和Log8-epi-PGF2α明显升高;(ii)I型糖尿病组唾液中的LogPC明显较对照组高,II型糖尿病组Log8-OHdG、LogPC和Log8-epi-PGF2α均较对照组有明显升高;(iii)二甲双胍治疗组和单纯餐饮控制的糖尿病组唾液Log8-OHdG和LogPC水平较高;(iv)单纯胰岛素治疗组和胰岛素及二甲双胍联合治疗组唾液LogPC水平高于对照组;(v)多变量分析和条件逻辑回归分析表明Log8-OHdG、LogPC、Log8-epi-PGF2α和糖尿病间存在明显的相关性。结论:糖尿病患者唾液中氧化性DNA、脂类和蛋白质损伤产物8-OHdG、8-epi-PGF2α、PC水平均明显升高,表明存在唾液中的氧化还原动态平衡紊乱。唾液氧胁迫指标的分析有可能成为一种新的疾病监测方式,并且提示一种疾病干预的新途径。
OBJECTIVE: To investigate whether diabetes is associated with changes in redox homeostasis in saliva. Methods: 8-hydroxy-2’-deoxyguanosine (8-OHdG), a marker of oxygen-induced damage in non-irritating saliva of 148 diabetic patients and 475 controls, The content of protein carbonyls (PC) and 8-epi-prostaglandin-F2α (8-epi-PGF2α) Logistic correlation analysis was used to analyze the correlation between the indicators of oxygen stress injury and the use of insulin, metformin and food control in patients with diabetes. Results: (i) Compared with the control group, Log8-OHdG, LogPC and Log8-epi-PGF2α in saliva of diabetic group were significantly increased; (ii) LogPC in saliva of type I diabetic patients was significantly higher than that of control group, (Iii) Log8-OHdG and LogPC levels in the saliva of metformin-treated and diet-controlled diabetic patients were higher than those in control group; (iv) Simple insulin therapy (V) Multivariate analysis and conditional logistic regression analysis showed that there was a significant correlation between Log8-OHdG, LogPC, Log8-epi-PGF2α and diabetes mellitus. Conclusion: The levels of 8-OHdG, 8-epi-PGF2α and PC in oxidative DNA, lipids and proteins in the saliva of diabetic patients were significantly increased, indicating that redox dynamic imbalance in saliva exists. The analysis of indicators of salivary oxygen stress is likely to become a new way of disease surveillance and suggests a new way of disease intervention.